The Next Generation Sequencing and its Effect on the Rice Molecular Design Breeding

首页当期上期下期 《分子植物育种》 ISSN:1672-416X CN: 46-1068/S 2008年第6卷第4期第619至630页

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· Zhou Degui

· Zhao Qiongyi

· Fu Chongyun

· Li Hong

· Cai Xuefei

· Luo Da

· Zhou Shaochuan

The Next Generation Sequencing and its Effect on the Rice Molecular Design Breeding

Zhou Degui ¹ Zhao Qiongyi ² Fu Chongyun ¹ Li Hong ¹ Cai Xuefei ² Luo Da ^2* Zhou Shaochuan ^1*

1 The Rice Research Institute of Guangdong Academy of Agricultural Sciences, Guangzhou, 510640
2 Shanghai Institutes of Plant Physiology and Ecology, CAS, Shanghai, 200032

摘要

This review is about the principle of the next generation sequencing and the history, workflow, advantages and cost of the four sequencing platforms, Roche (454) GS FLX sequencer, Illumina genome analyzer (solexa), Applied Biosystems SOLiD sequencer, HeliScope Sequencer, developed on the principle of Sequencing by Synthesis or Ligation. It was discussed the effect of new technology on rice molecular design breeding. It is suggested that the whole genome sequencing of a series of rice core germplasm, development of functional molecular markers and specific microarrys will accelerate the application of the basic research results to the breeding and lays the foundation for molecular design breeding.

关键词： Rice Next generation sequencing Molecular design breeding

DOI:10.3969/mpb.6.4.0619.630

语言： Chinese with English abstract

通讯作者：Luo Da dluo

sibs.ac.cn Zhou Shaochuan xxs123

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